Actinomycin

• Actonomycin D
• Natural product.
• intercalates into DNA
• Actinomycin D does this by binding DNA at the transcription initiation complex and preventing elongation of RNA chain by RNA polymerase.
• PDB: 173d
• 7-amino actinomycin D is a fluorescent analog probe compound useful for flow cytometry or staining
  • almost 100 nm Stokes shift
• for treatment of:
  • rhabdomyosarcoma
  • Wilm’s tumor (How is tumor different from cancer? Are there cancers that are not tumors? Are all tumors cancers?)

Questions

1. What are sarcomas?
2. How is tumor different from cancer?
3. Are there cancers that are not tumors?
4. Are all tumors cancers?
5. What is the Stokes shift?
6. Describe interactions of actinomycin D to double stranded DNA.